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steponeplus real time pcr system  (Thermo Fisher)


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    Thermo Fisher steponeplus real time pcr system
    Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/steponeplus+real-time+pcr+system/nct03277170-14-53-50
    Average 90 stars, based on 1 article reviews
    steponeplus real time pcr system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.
    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using the following cycling protocol: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of denaturation at 95 ◦C for 30 s, annealing at 60 ◦C for 30 s, and extension at 72 ◦C for 30 s. Changes in expression were determined using the 2−(∆∆Ct) method as described by Livak and Schmittgen [69].

    Article Title: Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function
    Article Snippet: Quantification of DNA fragments ranging from 25 bp to12,000 bp was carried out using ABI StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Article Title: Reactive oxygen species regulate adipose-osteogenic lineage commitment of human mesenchymal stem cells by modulating gene expression of C/EBP homology protein and aldo-keto reductase family 1 member A1
    Article Snippet: The following primers targeting the human Akr1A1 promoter region were used: Forward : 5’-GCCGACACCAGAAGAACC-3’ ; Reverse : 5’-CCTTTTGCTGGCCCACATTGC-3’ . qPCR was performed using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific Inc).

    Article Title: Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
    Article Snippet: Amplification was performed on an ABI StepOnePlus Real-Time PCR System (ABI, Germany) instrument with the following cycling protocol: initial denaturation at 95 °C for 2 min, followed by 40 cycles of denaturation (95 °C, 10 s) and annealing/extension (58 °C, 30 s).

    Article Title: Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
    Article Snippet: Subsequently, quantitative PCR was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).

    Article Title: Valproic acid exposure alters histone deacetylase mRNA expression profile in oral cancer and premalignant cell lines
    Article Snippet: The thermocycling conditions were 95 ̊C for 10 min (initial denaturation), followed by 45 cycles of 95 ̊C for 15 sec (denaturation) and 60 ̊C for 1 min (annealing and extension), on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis
    Article Snippet: A total of three replicates/sample were run for both TYMS and β-actin on the Applied Biosystems StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and the results were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific, Inc.). qPCR thermocycling conditions were as follows: Initial denaturation at 95 ̊C for 60 sec, followed by 40 cycles of denaturation at 95 ̊C for 15 sec and extension at 60 ̊C for 30 sec.

    Article Title: Expression of Olfactory Sense-Related Genes in Chicken Olfactory Epithelium
    Article Snippet: The PCR reactions were conducted under the following conditions: 50°C for 2 min, 95°C for 2 min, 45 cycles of 95°C for 15 s, 58.3–59°C for 15 s, and 72°C for 1 min, and a melting curve analysis at 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    Software:

    Article Title: Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.
    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using the following cycling protocol: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of denaturation at 95 ◦C for 30 s, annealing at 60 ◦C for 30 s, and extension at 72 ◦C for 30 s. Changes in expression were determined using the 2−(∆∆Ct) method as described by Livak and Schmittgen [69].

    Article Title: Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function
    Article Snippet: Quantification of DNA fragments ranging from 25 bp to12,000 bp was carried out using ABI StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Article Title: Reactive oxygen species regulate adipose-osteogenic lineage commitment of human mesenchymal stem cells by modulating gene expression of C/EBP homology protein and aldo-keto reductase family 1 member A1
    Article Snippet: The following primers targeting the human Akr1A1 promoter region were used: Forward : 5’-GCCGACACCAGAAGAACC-3’ ; Reverse : 5’-CCTTTTGCTGGCCCACATTGC-3’ . qPCR was performed using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific Inc).

    Article Title: Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
    Article Snippet: Amplification was performed on an ABI StepOnePlus Real-Time PCR System (ABI, Germany) instrument with the following cycling protocol: initial denaturation at 95 °C for 2 min, followed by 40 cycles of denaturation (95 °C, 10 s) and annealing/extension (58 °C, 30 s).

    Article Title: Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
    Article Snippet: Subsequently, quantitative PCR was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).

    Article Title: Valproic acid exposure alters histone deacetylase mRNA expression profile in oral cancer and premalignant cell lines
    Article Snippet: The thermocycling conditions were 95 ̊C for 10 min (initial denaturation), followed by 45 cycles of 95 ̊C for 15 sec (denaturation) and 60 ̊C for 1 min (annealing and extension), on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis
    Article Snippet: A total of three replicates/sample were run for both TYMS and β-actin on the Applied Biosystems StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and the results were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific, Inc.). qPCR thermocycling conditions were as follows: Initial denaturation at 95 ̊C for 60 sec, followed by 40 cycles of denaturation at 95 ̊C for 15 sec and extension at 60 ̊C for 30 sec.

    Article Title: Expression of Olfactory Sense-Related Genes in Chicken Olfactory Epithelium
    Article Snippet: The PCR reactions were conducted under the following conditions: 50°C for 2 min, 95°C for 2 min, 45 cycles of 95°C for 15 s, 58.3–59°C for 15 s, and 72°C for 1 min, and a melting curve analysis at 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    Amplification:

    Article Title: Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.
    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using the following cycling protocol: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of denaturation at 95 ◦C for 30 s, annealing at 60 ◦C for 30 s, and extension at 72 ◦C for 30 s. Changes in expression were determined using the 2−(∆∆Ct) method as described by Livak and Schmittgen [69].

    Article Title: Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function
    Article Snippet: Quantification of DNA fragments ranging from 25 bp to12,000 bp was carried out using ABI StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Article Title: Reactive oxygen species regulate adipose-osteogenic lineage commitment of human mesenchymal stem cells by modulating gene expression of C/EBP homology protein and aldo-keto reductase family 1 member A1
    Article Snippet: The following primers targeting the human Akr1A1 promoter region were used: Forward : 5’-GCCGACACCAGAAGAACC-3’ ; Reverse : 5’-CCTTTTGCTGGCCCACATTGC-3’ . qPCR was performed using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific Inc).

    Article Title: Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
    Article Snippet: Amplification was performed on an ABI StepOnePlus Real-Time PCR System (ABI, Germany) instrument with the following cycling protocol: initial denaturation at 95 °C for 2 min, followed by 40 cycles of denaturation (95 °C, 10 s) and annealing/extension (58 °C, 30 s).

    Article Title: Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
    Article Snippet: Subsequently, quantitative PCR was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).

    Article Title: Valproic acid exposure alters histone deacetylase mRNA expression profile in oral cancer and premalignant cell lines
    Article Snippet: The thermocycling conditions were 95 ̊C for 10 min (initial denaturation), followed by 45 cycles of 95 ̊C for 15 sec (denaturation) and 60 ̊C for 1 min (annealing and extension), on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis
    Article Snippet: A total of three replicates/sample were run for both TYMS and β-actin on the Applied Biosystems StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and the results were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific, Inc.). qPCR thermocycling conditions were as follows: Initial denaturation at 95 ̊C for 60 sec, followed by 40 cycles of denaturation at 95 ̊C for 15 sec and extension at 60 ̊C for 30 sec.

    Article Title: Expression of Olfactory Sense-Related Genes in Chicken Olfactory Epithelium
    Article Snippet: The PCR reactions were conducted under the following conditions: 50°C for 2 min, 95°C for 2 min, 45 cycles of 95°C for 15 s, 58.3–59°C for 15 s, and 72°C for 1 min, and a melting curve analysis at 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.
    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using the following cycling protocol: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of denaturation at 95 ◦C for 30 s, annealing at 60 ◦C for 30 s, and extension at 72 ◦C for 30 s. Changes in expression were determined using the 2−(∆∆Ct) method as described by Livak and Schmittgen [69].

    Article Title: Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function
    Article Snippet: Quantification of DNA fragments ranging from 25 bp to12,000 bp was carried out using ABI StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Article Title: Reactive oxygen species regulate adipose-osteogenic lineage commitment of human mesenchymal stem cells by modulating gene expression of C/EBP homology protein and aldo-keto reductase family 1 member A1
    Article Snippet: The following primers targeting the human Akr1A1 promoter region were used: Forward : 5’-GCCGACACCAGAAGAACC-3’ ; Reverse : 5’-CCTTTTGCTGGCCCACATTGC-3’ . qPCR was performed using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific Inc).

    Article Title: Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
    Article Snippet: Amplification was performed on an ABI StepOnePlus Real-Time PCR System (ABI, Germany) instrument with the following cycling protocol: initial denaturation at 95 °C for 2 min, followed by 40 cycles of denaturation (95 °C, 10 s) and annealing/extension (58 °C, 30 s).

    Article Title: Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
    Article Snippet: Subsequently, quantitative PCR was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).

    Article Title: Valproic acid exposure alters histone deacetylase mRNA expression profile in oral cancer and premalignant cell lines
    Article Snippet: The thermocycling conditions were 95 ̊C for 10 min (initial denaturation), followed by 45 cycles of 95 ̊C for 15 sec (denaturation) and 60 ̊C for 1 min (annealing and extension), on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis
    Article Snippet: A total of three replicates/sample were run for both TYMS and β-actin on the Applied Biosystems StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and the results were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific, Inc.). qPCR thermocycling conditions were as follows: Initial denaturation at 95 ̊C for 60 sec, followed by 40 cycles of denaturation at 95 ̊C for 15 sec and extension at 60 ̊C for 30 sec.

    Article Title: Expression of Olfactory Sense-Related Genes in Chicken Olfactory Epithelium
    Article Snippet: The PCR reactions were conducted under the following conditions: 50°C for 2 min, 95°C for 2 min, 45 cycles of 95°C for 15 s, 58.3–59°C for 15 s, and 72°C for 1 min, and a melting curve analysis at 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    SYBR Green Assay:

    Article Title: Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.
    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using the following cycling protocol: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of denaturation at 95 ◦C for 30 s, annealing at 60 ◦C for 30 s, and extension at 72 ◦C for 30 s. Changes in expression were determined using the 2−(∆∆Ct) method as described by Livak and Schmittgen [69].

    Article Title: Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function
    Article Snippet: Quantification of DNA fragments ranging from 25 bp to12,000 bp was carried out using ABI StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Article Title: Reactive oxygen species regulate adipose-osteogenic lineage commitment of human mesenchymal stem cells by modulating gene expression of C/EBP homology protein and aldo-keto reductase family 1 member A1
    Article Snippet: The following primers targeting the human Akr1A1 promoter region were used: Forward : 5’-GCCGACACCAGAAGAACC-3’ ; Reverse : 5’-CCTTTTGCTGGCCCACATTGC-3’ . qPCR was performed using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific Inc).

    Article Title: Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
    Article Snippet: Amplification was performed on an ABI StepOnePlus Real-Time PCR System (ABI, Germany) instrument with the following cycling protocol: initial denaturation at 95 °C for 2 min, followed by 40 cycles of denaturation (95 °C, 10 s) and annealing/extension (58 °C, 30 s).

    Article Title: Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
    Article Snippet: Subsequently, quantitative PCR was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).

    Article Title: Valproic acid exposure alters histone deacetylase mRNA expression profile in oral cancer and premalignant cell lines
    Article Snippet: The thermocycling conditions were 95 ̊C for 10 min (initial denaturation), followed by 45 cycles of 95 ̊C for 15 sec (denaturation) and 60 ̊C for 1 min (annealing and extension), on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis
    Article Snippet: A total of three replicates/sample were run for both TYMS and β-actin on the Applied Biosystems StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and the results were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific, Inc.). qPCR thermocycling conditions were as follows: Initial denaturation at 95 ̊C for 60 sec, followed by 40 cycles of denaturation at 95 ̊C for 15 sec and extension at 60 ̊C for 30 sec.

    Article Title: Expression of Olfactory Sense-Related Genes in Chicken Olfactory Epithelium
    Article Snippet: The PCR reactions were conducted under the following conditions: 50°C for 2 min, 95°C for 2 min, 45 cycles of 95°C for 15 s, 58.3–59°C for 15 s, and 72°C for 1 min, and a melting curve analysis at 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    Quantitative RT-PCR:

    Article Title: Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.
    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using the following cycling protocol: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of denaturation at 95 ◦C for 30 s, annealing at 60 ◦C for 30 s, and extension at 72 ◦C for 30 s. Changes in expression were determined using the 2−(∆∆Ct) method as described by Livak and Schmittgen [69].

    Article Title: Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function
    Article Snippet: Quantification of DNA fragments ranging from 25 bp to12,000 bp was carried out using ABI StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Article Title: Reactive oxygen species regulate adipose-osteogenic lineage commitment of human mesenchymal stem cells by modulating gene expression of C/EBP homology protein and aldo-keto reductase family 1 member A1
    Article Snippet: The following primers targeting the human Akr1A1 promoter region were used: Forward : 5’-GCCGACACCAGAAGAACC-3’ ; Reverse : 5’-CCTTTTGCTGGCCCACATTGC-3’ . qPCR was performed using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific Inc).

    Article Title: Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
    Article Snippet: Amplification was performed on an ABI StepOnePlus Real-Time PCR System (ABI, Germany) instrument with the following cycling protocol: initial denaturation at 95 °C for 2 min, followed by 40 cycles of denaturation (95 °C, 10 s) and annealing/extension (58 °C, 30 s).

    Article Title: Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
    Article Snippet: Subsequently, quantitative PCR was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).

    Article Title: Valproic acid exposure alters histone deacetylase mRNA expression profile in oral cancer and premalignant cell lines
    Article Snippet: The thermocycling conditions were 95 ̊C for 10 min (initial denaturation), followed by 45 cycles of 95 ̊C for 15 sec (denaturation) and 60 ̊C for 1 min (annealing and extension), on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis
    Article Snippet: A total of three replicates/sample were run for both TYMS and β-actin on the Applied Biosystems StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and the results were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific, Inc.). qPCR thermocycling conditions were as follows: Initial denaturation at 95 ̊C for 60 sec, followed by 40 cycles of denaturation at 95 ̊C for 15 sec and extension at 60 ̊C for 30 sec.

    Article Title: Expression of Olfactory Sense-Related Genes in Chicken Olfactory Epithelium
    Article Snippet: The PCR reactions were conducted under the following conditions: 50°C for 2 min, 95°C for 2 min, 45 cycles of 95°C for 15 s, 58.3–59°C for 15 s, and 72°C for 1 min, and a melting curve analysis at 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    Expressing:

    Article Title: Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.
    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using the following cycling protocol: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of denaturation at 95 ◦C for 30 s, annealing at 60 ◦C for 30 s, and extension at 72 ◦C for 30 s. Changes in expression were determined using the 2−(∆∆Ct) method as described by Livak and Schmittgen [69].

    Article Title: Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function
    Article Snippet: Quantification of DNA fragments ranging from 25 bp to12,000 bp was carried out using ABI StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Article Title: Reactive oxygen species regulate adipose-osteogenic lineage commitment of human mesenchymal stem cells by modulating gene expression of C/EBP homology protein and aldo-keto reductase family 1 member A1
    Article Snippet: The following primers targeting the human Akr1A1 promoter region were used: Forward : 5’-GCCGACACCAGAAGAACC-3’ ; Reverse : 5’-CCTTTTGCTGGCCCACATTGC-3’ . qPCR was performed using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific Inc).

    Article Title: Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
    Article Snippet: Amplification was performed on an ABI StepOnePlus Real-Time PCR System (ABI, Germany) instrument with the following cycling protocol: initial denaturation at 95 °C for 2 min, followed by 40 cycles of denaturation (95 °C, 10 s) and annealing/extension (58 °C, 30 s).

    Article Title: Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
    Article Snippet: Subsequently, quantitative PCR was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).

    Article Title: Valproic acid exposure alters histone deacetylase mRNA expression profile in oral cancer and premalignant cell lines
    Article Snippet: The thermocycling conditions were 95 ̊C for 10 min (initial denaturation), followed by 45 cycles of 95 ̊C for 15 sec (denaturation) and 60 ̊C for 1 min (annealing and extension), on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis
    Article Snippet: A total of three replicates/sample were run for both TYMS and β-actin on the Applied Biosystems StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and the results were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific, Inc.). qPCR thermocycling conditions were as follows: Initial denaturation at 95 ̊C for 60 sec, followed by 40 cycles of denaturation at 95 ̊C for 15 sec and extension at 60 ̊C for 30 sec.

    Article Title: Expression of Olfactory Sense-Related Genes in Chicken Olfactory Epithelium
    Article Snippet: The PCR reactions were conducted under the following conditions: 50°C for 2 min, 95°C for 2 min, 45 cycles of 95°C for 15 s, 58.3–59°C for 15 s, and 72°C for 1 min, and a melting curve analysis at 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).



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